Phosphatase Assay

Phosphatase Assay

The Phosphatase Assay kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases.


The Phosphatase Assay kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases (Figure).


The phosphatases remove the phosphate group to generate p-nitrophenol, which is deprotonated under alkaline conditions to produce p-nitrophenolate that has strong absorption at 405nm.


The kits components are sufficient for performing up to 1000 assays in 96-well plate format and easily adaptable to cuvettes or 384-well plates.



  • A colorimetric, pNPP based assay.
  • Measure phosphatase activity in biological samples.
  • Screen for phosphatase agonists and inhibitors.



  •  For the quantification of phosphatase activity.
  •  To screen for agonists and inhibitors of phosphatases.


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Other Technical Literatures Description
Plant Proteomics Handbook
Protein Assay Handbook & Selection Guide  An introduction to protein assays.
Bioassays Handbook
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
Catalog Description Size Price(USD) Qty
Phosphatase Assay kit 1,000 Assays