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Plasmid Screening Reagents

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HP Ampicillin™ is a stronger and more stable inhibitor of ß-lactamase than regular ampicillin and, therefore, supports only the growth of ampicillin resistant colonies even after prolonged incubation.   Culture plates can be incubated for a longer period at 37°C, eliminating t..
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Gene/Protein:
Plasmid Screening Toothpick™ is a unique system that allows for the rapid screening of bacteria for transformed plasmids, without the need for an overnight culture. Pick a colony, add to Plasmid Screening Toothpick™ reagents and then analyze using restriction enzymes.   ..
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Gene/Protein:
Toothpick™-PCR is an extension of Toothpick™ and allows for the rapid release of plasmids from transformed bacteria for screening by polymerase chain reaction (PCR). There is no requirement for growing bacteria, performing "minipreps" or purifying the plasmid DNA.   ..
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Gene/Protein:
IPTG (Isopropyl β-D-1-thiogalactopyranoside) is a useful molecular biology reagent. IPTG is a molecular mimic of allolactose that initiates transcription of the lac operon.  The key feature of IPTG is that, unlike allolactose, is not hydrolyzed by ß-galactosidase, therefore its ..
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