2X Taq Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. The mix retains all features of Taq DNA Polymerase.
Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb. The elongation velocity is 0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product
One unit (U) of Taq polymerase is defined as the amount of enzyme needed to catalyze the incorporation of 10 nanomoles of
deoxyribonucleotides into acid-insoluble material in 30 minutes at 74°C.
COMPOSITION OF THE TAQ MIX
Taq DNA polymerase is supplied in 2X PCR buffer, dNTPs, 3.2mM MgCl4 and 0.02% bromophenol blue. Taq mix buffer is a proprietary formulation optimized for robust performance in PCR.
Individual Taq DNA polymerase and Pfu DNA polymerase are also available.
- Convenient –Taq DNA Polymerase in a ready-to-use mix.
- High yields of PCR products with minimal optimization.
- Fast -saves time due to reduced number of pipetting steps.
- Reproducible -lower contamination and pipetting error risk.
- High throughput PCR.
- Routine PCR with high reproducibility
- Generation of PCR products for TA cloning