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PopLysis™ Mammalian Lysis and Extraction Buffer

PopLysis_Mammalian_Lysis_and_Extraction_Buffer

PopLysis™ Mammalian Lysis and Extraction buffer is part of our PopLysis™ buffer systems. PopLysis™ buffers are based on cocktail of membrane solubilizing detergents optimized to rapidly solubilize and pop open membrane structures allowing cellular proteins to spill out into lysis solution. Mammalian cells solubilize immediately releasing entire cellular components as soon as PopLysis™ Mammalian Lysis and Extraction buffer come in contact with cells. No mechanical force is required for 100% solubilization and recovery of cellular proteins.

Features

  • Total protein extraction: Extract membrane and cytosolic proteins
  • Rapid: Cell lysis takes place within 10 -15 minutes.
  • Gentle and non-dentauring: Buffer is mild and does not affect affect the function of extracted proteins.
  • Amine free formulation
  • Suitable for both adherent and suspension mammalian cells

Applications

  • PopLysis™ Mammalian Lysis and Extraction buffer is mild buffer for extraction of total (membrane and cytosolic) protein extraction
Protocol
786-1673

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