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TCM ProteaseArrest™ Protease Inhibitor Cocktail (1 Citations)

Tissue Culture Media Protease Inhibitor Cocktail

A broad range, 200X concentrated, sterile filtered, ready-to-use protease inhibitor cocktail for the protection of proteins secreted into tissue culture media. TCM ProteaseArrest™ features optimized concentrations of Aprotinin, Bestatin, E-64, Leupeptin and Pepstatin A that inhibits a wide range of serine, cysteine and other specific proteases including aminopeptidases  trypsin-like and acid proteases. The inhibitor cocktail is designed to protect secreted proteins during cell culture for up to 48 hours.

Sample Type & key features

Inhibition Specificity

1X Composition

Concentration & Available Sizes

For protection of secreted proteins in tissue culture media.

Sterile filtered

A wide range of serine, cysteine and other specific proteases including aminopeptidases trypsin-like and acid proteases

0.6µM Aprotinin

58µM Bestatin

28µM E-64

10.5µM Leupeptin

2.1µM Pepstatin A

200X Sterile filtered DMSO solution

Features

  • No proprietary ingredients, fully disclosed formulation of five broad range protease inhibitors, including Aprotinin, Bestatin, E-64, Leupeptin, Pepstatin A
  • Broad range protease inhibitor cocktail
  • Stable for up to 48 hours in cell culture media
  • Sterile filtered

Applications

  • Protect secreted proteins from proteolytic degradation during cell culture 

ProteaseArrest Family

Our ProteaseArrest™ is a highly efficient, liquid format general protease inhibitor cocktail that offers >95% protease inhibition. The ProteaseArrest™ family of inhibitor cocktails includes other species-specific inhibitor cocktails as well, all of which are enhanced with inhibitors specific for that species.  In addition to Bacteria, species-specific cocktails are available for Bacterial, Plant, Yeast, Fungus and Mammalian samples. Our Recom ProteaseArrest™ is designed for recombinant protein purification, particularly 6X His tagged proteins. These proteins are purified by IMAC (Immobilized Metal Affinity Chromatography), which is severely inhibited by the routinely used metalloproteases inhibitor EDTA. Recom ProteaseArrest™ contains a specific bacterial metalloproteases that does not interfere with 6X His protein purification. Our FOCUS™ ProteaseArrest™ is designed to protect proteins destined for 2D electrophoresis and mass spectrometry analysis. 

Protocol
786-238
786-239
Material Safety Data Sheet
786-238
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786-239
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish

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TCM ProteaseArrest™ Protease Inhibitor Cocktail
TCM ProteaseArrest™ Protease Inhibitor Cocktail
A broad range, 200X concentrated, sterile filtered, ready-to-use protease inhibitor cocktail for the protection of proteins secreted into tissue cultu..
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