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FOCUS™ ProteaseArrest™

Catalog Description Size Price(USD) Qty
786-108F
FOCUS™ ProteaseArrest™ [100X] 1ml
$168.00

FOCUS™ ProteaseArrest™

FOCUS™ ProteaseArrest™ is a ready-to-use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry.
 
The protease inhibitor cocktail contains reversible and irreversible inhibitors of serine, cysteine, calpain and metallo- proteases. Due to the optimized concentration of the various inhibitors, the FOCUS™ ProteaseArrest™ shows excellent inhibition of protease activities, and is therefore suitable for the protection of protein samples from animal tissues, plants, yeast and bacteria.
 
FOCUS™ Protease Arrest™ is compatible with 2D electrophoresis as it uses an alternative to EDTA as an inhibitor of metalloproteases. The absence of EDTA allows for optimal action of nucleases or removing nucleic acids from the samples. In addition, FOCUS™ ProteaseArrest™ uses PMSF as its primary serine protease inhibitor as opposed to the commonly used Pefabloc®. Pefabloc® has been reported to modify proteins at high concentrations and result in artifacts in subsequent 2D electrophoresis and mass spectrometry.
 

ProteaseArrest Family

Our ProteaseArrest™ is a highly efficient, liquid format general protease inhibitor cocktail that offers >95% protease inhibition.

 

The ProteaseArrest™ family of inhibitor cocktails also includes species specific inhibitor cocktails.  These cocktails are enhanced with inhibitors specific for the species.  Species specific cocktails are available for BacteriaPlantYeast, Fungus and Mammalian samples.

 

Our Recom ProteaseArrest™ is designed for recombinant protein purification, particularly 6X His tagged proteins. These proteins are purified by IMAC (Immobilized Metal Affinity Chromatography), which is severely inhibited by the routinely used metalloproteases inhibitor EDTA. Recom ProteaseArrest™ contains a specific bacterial metalloproteases that does not interfere with 6X His protein purification.

 


Features

  •  2D electrophoresis compatible, broad spectrum protease inhibitor cocktail.
  •  100X concentrated, ready-to-use solution.
  •  High inhibition levels: 1X FOCUS™ ProteaseArrest™ inhibits >95% of protease activities (i.e. Mouse Pancreas Extract, 0.5mg/ml protein).

 


Applications

  •  Inhibition of protease activity in protein preparations.
  •  Suitable for 2D gel sample preparation for protection of protein samples.

 

 

Protocol
786-108F
Material Safety Data Sheet
786-108F
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Technical Literature
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
ProteaseArrestâ„¢ Protease Inhibitor Cocktail Offers Over 90% Inhibition of Proteases Improved protease inhibition compared to tablet cocktails  
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
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