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Protease Inhibitors

G-Biosciences offer an extensive range of protease inhibitor cocktails and ready-to-use protease inhibitors. Our ProteaseARREST™ line of protease inhibitor cocktails are highly potent, liquid format cocktails that are ready-to-use, highly stable and eliminate waste. These cocktails are suitable for protein purification when added to cell lysis and protein extraction buffers:

  • ProteaseARREST™ is a broad spectrum, general use, 100X concentrated protease inhibitor cocktail. The cocktail contains a mix of reversible and irreversible inhibitors of serine, cysteine, calpain and metallo-proteases. The 1X composition is 668µM AEBSF, 0.3µM Aprotinin, 3µM Bestatin, 5.25µM Leupeptin and 1mM PMSF in DMSO with optional 5mM EDTA. Supplied as a 100X DMSO solution in 24 x 100µl, 2ml, 5ml, 10ml and 5 x 10ml vials.
  • Species Specific ProteaseARREST™ are 100X concentrated protease inhibitor cocktails and feature the following:
    • Bacterial ProteaseARREST™ for bacterial cell extracts. A 100X concentrated protease inhibitor cocktail featuring bacterial serine, cysteine and other bacterial specific proteases including aminopeptidases and aspartic proteases. The 1X composition is 668µM AEBSF, 3µM Bestatin, 14µM E-64, 1µM Pepstatin A and 1mM PMSF in DMSO with optional 5mM EDTA. Supplied as a 100X DMSO solution in 1ml and 5ml vials.
    • Mammalian ProteaseARREST™ for mammalian cells and tissue extracts. A 100X concentrated protease inhibitor cocktail featuring mammalian serine, cysteine and other mammalian specific proteases including aminopeptidases, trypsin-like and aspartic proteases. The 1X composition is 668µm AEBSF, 0.3µM Aprotinin, 3µM Bestatin, 14µM E-64, 5.25µM Leupeptin, 1µM Pepstatin A and 1mM PMSF in DMSO with optional 5mM EDTA. Supplied as a 100X DMSO solution in 1ml and 5ml vials.
    • Plant ProteaseARREST™ for plant cells and tissue extracts. A 100X concentrated protease inhibitor cocktail featuring plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases. The 1X composition is 668µM AEBSF, 3µM Bestatin, 14µM E-64, 5.25µM Leupeptin, 1µM Pepstatin A, 2mM 1,10-Phenathroline and 1mM PMSF in DMSO. Supplied as a 100X DMSO solution in 1ml and 5ml vials.
    • Yeast/Fungal ProteaseARREST™ to inhibit yeast and fungal serine, cysteine and metalloproteases. The 1X composition is 668µM AEBSF, 14µM E-64, 1µM Pepstatin A, 2mM 1,10-Phenathroline and 1mM PMSF in DMSO. Supplied as a 100X DMSO solution in 1ml and 5ml vials.
  • Recom ProteaseARREST™ is broad range, bacterial, 100X concentrated, ready-to-use protease inhibitor cocktail specifically designed for use in the purification of recombinant proteins from bacteria and is fully compatible with His tag protein purification with IMAC. The protease inhibitor cocktail inhibits bacterial serine, cysteine, metallo- and other bacterial specific proteases including aminopeptidases and aspartic proteases. The 1X composition is 668µM AEBSF, 1mM 1,10-Phenathroline, 1mM Benzamidine, 1mM Iodoacetamide, 1µM Pepstatin A  and 1mM PMSF in DMSO. Supplied as a 100X DMSO solution in 1ml and 5ml vials.
  • TCM ProteaseARREST™ is broad range, 200X concentrated, ready-to-use protease inhibitor cocktail that is sterile filtered for tissue culture media (TCM). The inhibitor cocktail is designed to protect secreted proteins during cell culture for up to 48 hours. The 1X composition is 0.6µM Aprotinin, 58µM Bestatin, 28µM E-64, 10.5µM Leupeptin and 2.1µM Pepstatin A  in DMSO. Supplied as a 200X sterile filtered DMSO solution in 1ml and 2ml vials.
  • FOCUS™ ProteaseARREST™ is a highly potent protease inhibitor designed for sensitive downstream proteomic analysis, including 2D and mass spec. The use of an alternative metalloprotease to EDTA allows for optimal action of nucleases to remove interfering nucleic acids and has an alternative to AEBSF (Pefabloc®) that has been shown to result in artifacts in mass spec. The 1X composition is 340µM AEBSF, 0.3µM Aprotinin, 1.45µM Bestatin, 3.4µM Calpain Inhibitor I, 5.25µM Leupeptin, 17µM Phosphoramidon and 1mM PMSF in DMSO. Supplied as a 100X DMSO solution in a 1ml vial.

Our ProteCEASE™ protease inhibitor cocktail line are dry format cocktail designed for extended shelf life and one shot additions to 50 and 100ml lysis buffer.  The ProteCEASE™ are supplied with or without EDTA and with a ProteCEASE™ buffer to resuspend the protease inhibitors. The 1X composition is 668µM AEBSF, 0.3µM Aprotinin, 3µM Bestatin, 5.25µM Leupeptin and 1mM PMSF in DMSO with optional 5mM EDTA. Supplied as single, 10 or 20 vials suitable for 50 or 100ml lysis buffer.

Download the Protease & Phosphatase Inhibitors, Enzymes & Assays Hanbook
Calpain inhibitor I; N-[N-(N-Acetyl-L-leucyl)-L-leucyl]-L-norleucine   Specificity: Cell permeable peptide aldehyde inhibitor of calpain I and to a lesser extent calpain II.  Also inhibits other neutral cysteine proteases, cathepsin B and L and the proteasome.   Solubility: Solubl..
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[(S)-1-Carboxy-2-Phenyl]-carbamoyl-Arg-Val-arginal   Specificity:  Inhibits Ca2+-dependent endopeptidases, including papain, trypsin-like serine proteases, some cysteine proteases and to a lesser extent plasmin.  Higher specificity for trypsin and papain compared to leupeptin. &nb..
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Also known as bovine pancreatic trypsin inhibitor.     Activity: Approx 6,000 KIU/mg   Specificity:  A broad range, competitive and reversible inhibitor of chymotrypsin, plasmin, trypsin, kallikrein and other serine proteases     Solubility: Soluble in water (Stoc..
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N-[(2S,3R)-3-Amino-2-hydroxy-4-phenylbutyryl]-L-leucine hydrochloride     Specificity: Competitive inhibitor of surface aminopeptidases, including aminopeptidase B (Ki=2nM), leucine aminopeptidase (Ki=20nM). Also inhibits aminopeptidases N; does not inhibit endoproteases.     ..
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N-[(S)-1-carboxy-isopentyl)-carbamoyl-alpha-(2-iminohexahydro-4(S)-pyrimidyl]-L-glycyl-L-phenylalaninal     Specificity: Inhibits serine proteases having a chymotrypsin-like specificity, including α, β γ, and δ chymotrypsin, and most cysteine proteases including ca..
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E-64 L-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane or N-[N-(L-trans-carboxyoxiran-2-carbonyl)-L-leucyl]-agmatine   Features CAS Number: 66701-25-5 Molecular Formula : C₁₅H₂₇Nâ‚…Oâ..
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EDTA disodium salt is a reversible metalloprotease inhibitor that is used for chelating metal ions. A 0.5M solution is also available.   Chemical Name: EDTA (Ethylenediaminetetracetic acid)   Synonyms: disodium ethylenediaminetetraacetate dihydrate; ethylenediamine tetraacetic acid d..
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Features Specificity: Inhibits serine, plasmin, porcine kallikrein and cysteine proteases, including papain and cathepsin B. Does not inhibit chymotrypsin and thrombin. Molecular weight: 475.59 Purity: Three tautomeric isomers in solution Solubility: Soluble in H2O (50mg/ml..
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Isovaleryl-Val-Val-AHMHA-Ala-AHMHA where AHMHA= (3S, 4S)-4-amino-3-hydroxy-6-methyl-heptanoic acid   Specificity: A potent inhibitor of various aspartic proteases, including cathepsin D, renin, pepsin, bacterial aspartic proteases and HIV proteases.   Solubility: Soluble in DMSO or met..
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N-alpha-L-rhamnopyranosyloxy(hydroxyphosphinyl)-L-Leucyl-L-Tryptophan   Specificity: Inhibits some metalloproteases, including thermolysin, collagenase and bacterial metallo proteases from Bacillus subtilis, Streptomyces griseus and Pseudomonas aeruginosa (metallo elastase).   Solubili..
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Features Synonyms: α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, Phenylmethylsulfonyl fluoride CAS Number: 329-98-6 Molecular Formula: C₇H₇FOâ‚‚S Molecular Weight: 174.19 g/mol Activity: >99.9% Solubility: Soluble in..
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Aprotinin is a monomeric globular protein which inhibits several serine proteases including trypsin, chymotrypsin, plasmin and kallikrein. Furthermore, its action on kallikrein leads to inhibition of formation of factorXIIa.The binding of aprotinin to proteases is reversible with dissociation of apr..
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