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SG-Lysine-C™

Catalog Description Size Price(USD) Qty
786-14
SG-Lysine-C™ 1 x 20µg
$272.00

SG-Lysine-C™

SG-Lysine-C endopeptidase, from Lysobacter enzymogenes, is a serine protease highly specific in cleaving peptide bonds at the carboxy side of lysine. Highly purified preparations of SG-Lysine-C are chemically modified making the enzyme resistant to autolysis and stabilizing its enzymatic activity.

 

SG-Lysine-C is supplied lyophilized in 20μg vials. The enzyme is typically reconstituted to a concentration of 0.25μg/ml. For fragmentation, the enzyme is added to the sample protein in a ratio of 1:100 to 1:20 (enzyme to protein, by weight) in a standard digestion buffer.

 


Features

  • Modified Lysine-C for sequence analysis.
  • Resistant to autolysis and degradation.
  • High Specificity, free from other known endopeptidases.
  • Consistency of activity levels from lot to lot for reproducible digestions.
  • Recommended SG-Lysine-C™ to protein ratio, 1:100 to 1:20.

 


Applications

  • Digestion of proteins for sequence analysis.
  • Suitable for sequencing applications.
Protocol
786-14
Material Safety Data Sheet
786-14
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Technical Literature
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
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