Papain is a cysteine protease enzyme (EC 188.8.131.52) that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. This cleavage results in the generation of three ~50kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonization, and lysis.
The Fab and Fc fragments can be further separated with either Immobilized Protein A or ion exchange chromatography.
Immobilized Papain is a convenient reagent for producing Fab and Fc fragments as it avoids the need to remove the papain enzyme after digestion.
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- Generate Fc and Fab from IgG
- Eliminates contamination with papain enzyme
- Can be used in virtually all scenarios using free papain