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Trypsin, Mass Spectrometry Grade (50 Citations)

Trypsin
Mass Spec Grade Trypsin Stability
Mass Spec Grade Trypsin Activity in denaturing buffers
Trypsin
Mass Spec Grade Trypsin Stability
Mass Spec Grade Trypsin Activity in denaturing buffers






A Chemically Modified, TPCK treated, Affinity Purified Trypsin. G-Biosciences' Mass Spectrometry Grade Trypsin is chemically methylated to yield an enzymatically active protein with maximum trypsin specificity and extreme resistance to autolysis. In addition, the modified trypsin is TPCK treated to inactive the interfering chymotrypsin activity, and the resulting protein is affinity purified and lyophilized to produce our Mass Spectrometry Grade Trypsin. The resulting trypsin has a specific activity over 10,000 units per milligram protein.  The maximum activity is in the pH range of 7 to 9 and activity is reversibly inactivated at a pH of 4.

Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues, and typically, there is little to no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry. Native trypsin is prone to autolysis that results in pseudotrypsin, which exhibits a broader proteolytic specificity (a chymotrypsin-like activity) and trypsin fragments that interfere with sequence analysis.

Unlike other trypsin preparations, our Mass Spectrometry Grade Trypsin is highly stable.  As a result, it can be stored for a long period of time without any loss of activity (See figure above)).

We supply two sources of Mass Spectrometry Grade Trypsin, either bovine or porcine. For mass spectrometry sequence analysis, a trypsin to protein ratio ranging from 1:20 to 1:100 is recommended. For convenience, our porcine Mass Spectrometry Grade Trypsin is supplied lyophilized as 20, 100 or 200µg vials with a specific resuspension buffer. Our bovine Mass Spectrometry Grade Trypsin is supplied lyophilized as 20µg vials with a specific resuspension buffer.

Features

  • Mass Spec Grade Trypsin
  • Ultra pure porcine or bovine trypsin (A recombinant human trypsin is also offered)
  • Modified by methylation and TPCK treatment
  • Resistant to autolysis and degradation
  • Stable at ambient temperature and suitable for long term storage
  • Specific activity: >10,000U/mg protein

Applications

  • Digestion of proteins for sequence and peptide fragment analysis
  • Suitable for sequencing and mass spectrometry applications
Protocol
786-245
786-687
786-690
786-688
786-693
786-245B
786-687B
Material Safety Data Sheet
786-245
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-687
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-690
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-688
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-693
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-245B
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-687B
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays

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