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Streptavidin, recombinant

Recombinant Streptavidin
Catalog Description Size Price(USD) Qty
786-584
Streptavidin 5mg
$107.10 
$126.00
786-585
Streptavidin 25mg
$476.00 
$560.00
786-586
Streptavidin 100mg
$1,042.95 
$1,227.00

Streptavidin, recombinant

Recombinant streptavidin from streptomyces avidinii, produced in E.coli. Lyophilized in 20mM potassium phospate, pH6.5 and available in 5, 25 and 100mg.

Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for streptavidin (Ka=1015M-1). Biotin and streptavidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-streptavidin bonds can only be reversed by denaturing the streptavidin protein molecule with 8M guanidine-hydrochloride at pH1.5 or by autoclaving.

Streptavidin is a tetrameric protein that has no carbohydrate modifications. The solubility of streptavidin (isoelectric pH5) in aqueous buffer is lower than avidin, but the binding of streptavidin to biotin is similar to that of avidin. The advantage of streptavidin is that the lack of carbohydrates significantly reduces the amount of non-specific binding.

Streptavidin is also available immobilized on agarose and coated on 96-well plates

Features

  • White lyophilizate, lyophilized in 20mM potassium phosphate, pH6.5
  • Molecular weight: ~52,000Da
  • pH stability: pH 5.0-9.0
  • Solubility: 10mg/ml in water
  • Isoelectric point: 6.8-7.5
  • KD: 10-15M
  • Purity: Single band by SDS-PAGE
Material Safety Data Sheet
786-584
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786-585
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-586
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
Technical Literature
Protein Labeling & Conjugation
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