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Superior™ Blocking Buffer

Catalog Description Size Price(USD) Qty
786-601
Superior Blocking Buffer-Dry Blend in PBS 5 Packs
$144.00
786-655
Superior™ Blocking Buffer for Precipitating Substrate in TBS (Tris-buffered saline, pH 7.5) 500ml
$85.00
786-656
Superior™ Blocking Buffer for Precipitating Substrate in PBS (Phosphate-buffered saline, pH 7.5) 500ml
$85.00
786-657
Superior™ Blocking Buffer-Dry Blend in TBS (Tris-buffered saline, pH 7.5) 5 Packs
$144.00
786-658
Superior™ Blocking Buffer in TBS (Tris-buffered saline, pH 7.5) 500ml
$92.00
786-659
Superior™ Blocking Buffer in TBST (Tris-buffered saline, pH 7.5 with 0.05% Tween®-20) 500ml
$100.00
786-660
Superior™ Blocking Buffer in PBS (Phosphate-buffered saline, pH 7.5) 500ml
$92.00
786-661
Superior™ Blocking Buffer in PBST (Phosphate-buffered saline, pH 7.5 with 0.05% Tween®-20) 500ml
$100.00

Superior™ Blocking Buffer

Superior™ Blocking Buffer contains a proprietary antiginically non-determinant protein for blocking non-specific sites during ELISA, membrane blotting, immunohistochemistry and other applications. 

 

Superior™ Blocking Buffer is ideal for a high signal to background ratio in most system. Superior™ Blocking Buffer uses a non-serum protein and does not contain biotin or other animal source proteins to interfere with immuno-complexes. Superior™ Blocking Buffer is suitable for assays that use avidin/streptavidin systems.

 

Superior™ Blocking Buffer for Precipitating Substrate is a modification of Superior™ Blocking Buffer. This blotting buffer has been optimized for used in blotting protocols that use precipitating substrates, such as TMB (3, 3', 5, 5'-Tetramethylbenzidine) and BCIP (5-Bromo-4-Chloro-3'-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrates. Superior™ Blocking Buffer for Precipitating Substrate is not suitable for ELISA or immunohistochemistry staining.

 

Available in multiple formats using TBS, PBS, TBS with 0.05% Tween®-20 or PBS with .05% Tween®-20.  Also supplied as a convenient dry form that is stable at room temperature. Each dry format pack is makes 200ml Superior™ Blocking Buffer.

 


Features

  • Non serum protein blocking agent
  • Rapid blocking times; ~2 minutes for ELISA
  • Multiple formats; ready-to-use liquid (500ml) or dry buffer packs (for 5 x 200ml)
  • Available with and without detergent (Tween®-20)

 


Applications

  • For blocking Western blot membranes (PVDF and nitrocellulose)
  • For blocking and storage of ELISA plates
  • For blocking prior to immunohistochemistry staining

 

SuperiorBlocking Buffer for Precipitating Substrate is not suitable for ELISA or immunohistochemistry staining.

Protocol
786-601
786-655
786-656
786-657
786-658
786-659
786-660
786-661
Material Safety Data Sheet
786-601
English_US
Danish
Dutch
English_UK
French
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786-655
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-656
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-657
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-658
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-659
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-660
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-661
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Assay Development A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.
Bioassays Handbook
Western Blotting Handbook From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.
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