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TBST [10X] (2 Citations)



 A 10X Concentrated solution of Tris Buffered Saline with Tween® 20 with a concentration of 100mM Tris.HCl, 150mM NaCl, 0.5% Tween® 20 at pH7.5. The 1X Concentration is 10mM Tris.HCl, 15mM NaCl, 0.05% Tween® 20 at pH7.5. A dry buffer pack format is also avaialble to produce 1L of 1X TBST.

TBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. The Tris Buffered Saline with Tween® 20 is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from membranes and microtiter plate wells without disrupting the antigen/antibody binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.

Features

  •  1X Concentration is 10mM Tris.HCl, 15mM NaCl, 0.05% Tween® 20 at pH7.5

Applications

  •  Wash buffer for Western Blotting and ELISA
Material Safety Data Sheet
R042
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R043
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish

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