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TBST [10X]

Catalog Description Size Price(USD) Qty
R042
TBST [10X]; Tris buffered saline with Tween-20; 100mM Tris.HCl, 1.5M NaCl, 0.5% Tween-20, pH 7.5 1L
$56.00
R043
TBST [10X]; Tris buffered saline with Tween-20; 100mM Tris.HCl, 1.5M NaCl, 0.5% Tween-20, pH 7.5 1 Gallon
$175.00

TBST [10X]

 A 10X Concentrated solution of Tris Buffered Saline with Tween® 20. 

 

The 10X Concentration is 100mM Tris.HCl, 150mM NaCl, 0.5% Tween® 20 at pH7.5.

 

The 1X Concentration is 10mM Tris.HCl, 15mM NaCl, 0.05% Tween® 20 at pH7.5.

 

TBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. The Tris Buffered Saline with Tween® 20 is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from membranes and microtiter plate wells without disrupting the antigen/antibody binding reaction.

 

By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.

 


Features

  •  1X Concentration is 10mM Tris.HCl, 15mM NaCl, 0.05% Tween® 20 at pH7.5

 


Applications

  •  Wash buffer for Western Blotting and ELISA

 

 

Material Safety Data Sheet
R042
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R043
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
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