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2D-Detergent™ Triton® X-100


Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and carbonyls. The peroxides will oxidize proteins and the carbonyl groups will form Schiff's bases with the proteins that interfere with a protein's structure.

 


Our sterile 2D-Detergent solutions contain reduced peroxides and carbonyl compounds (Figure). In addition, the detergents have less than 15µS conductivity. These detergents are offered as 10% aqueous solutions, sealed under inert gas and are suitable for all protein applications, including 2D-electrophoresis. These non-ionic detergents are suitable for isolating membrane-protein complexes.
 

 


The aldehyde and peroxide levels are <50µM and <10µM respectively with a conductivity of <15µS.

 


Features

  • Low conductivity; <15µS
  • Low peroxide contamination.
  • Low carbonyl contamination.
  • Reduced metal ions.
  • Sterile detergent solution
  • 10% aqueous solutions.
  • Sealed under inert gas to prevent oxidation.

 

 

Material Safety Data Sheet
DG907
English_US
Danish
Dutch
English_UK
French
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DG908
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Detergent Handbook & Selection Guide A Comprehensive Guide to the Properties and Uses of Detergents in Research. Also features information on detergent removal systems.
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Certificate Of Analysis
DG907
090311
102211
110408
180811
180811-mod
DG908
180811
180811-mod
lot specific

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