The InGel™ Blue kit provides a complete set of reagents for the in gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie or fluorescent stained protein spots/bands.
The kit has all the reagents for the in-gel digestion of proteins by the supplied, chemically modified Mass Spectrometry Grade Trypsin. Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues. Typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds.
Trypsin naturally undergoes autolysis, producing trypsin fragments that interfere with sequence analysis. Our Mass Spectrometry Grade Trypsin is a chemically modified trypsin that is enzymatically active and yet resistant to autolysis. It is methylated, TPCK treated and quality tested for mass spectrometry.
Following trypsin digestion the kit allows for the extraction of the digested proteins from the gel pieces for analysis by mass spectrometry (MALDI and LC MS/MS). The InGel™ Blue kit is suitable for ~100 protein spots or bands.
For silver stained proteins, we recommend InGel™ Silver
- For the in-gel tryptic digestion of proteins
- Compatible with Coomassie and fluorescent stained proteins
- Suitable for MALDI peptide mapping and LC MS/MS
- Supplied with Mass spectrometry grade trypsin
- Supplied with destining reagents and peptide extraction reagents
- For MALDI Peptide Mass Mapping
- For LC MS/MS