Immobilized Papain (3 Citations)
Papain is a cysteine protease enzyme (EC 184.108.40.206) that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. This cleavage results in the generation of three ~50kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonization, and lysis.
The Fab and Fc fragments can be further separated with either Immobilized Protein A or ion exchange chromatography.
Immobilized Papain is a convenient reagent for producing Fab and Fc fragments as it avoids the need to remove the papain enzyme after digestion.
- Generate Fc and Fab from IgG
- Eliminates contamination with papain enzyme
- Can be used in virtually all scenarios using free papain
|Material Safety Data Sheet|
|Protease & Phosphatase Inhibitors & Proteases Handbook||A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays|