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Antibody Production & Purification

 

The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. The most common carrier proteins are bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH).

 

We offer a wide variety of carrier proteins for the successful generation of antibodies.Our ActiveHOOKproducts include both ActiveHOOK BSA (maleimide activated bovine serum albumin) and ActiveHOOKKLH (maleimide activated keyhole limpet hemocyanin). Both rapidly couple to free sulfhydryl groups on peptides and proteins using just one simple step. These activated carrier proteins save both time and money as separate cross-linkers are not required. Our HyperCarrier uses cationized BSA which has been found to elicit greater immunogenic response compared to normal BSA.

 

HOOK™  kits are also available for the coupling of peptides to carrier proteins. Both amine reactive and sulfhydryl reactive HOOKPeptide Coupling Kits are offered.

 

Download the Antibody Production Handbook for Complete Product Listing

An important tool in research is the generation of peptide and protein affinity columns for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offers a complete kit for the coupling of peptides and proteins to agarose through thei..
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Simple Antibody Biotinylation   The HOOK™ IgG Biotinylation (Sulfhydryl) kit is designed for the efficient biotinylation of IgG molecules by immobilizing the IgG molecules on a solid support (Figure 1).    IgG molecules contain a histidine rich region in their Fc region and ..
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Simple Antibody Biotinylation   The HOOK™ IgG Biotinylation (Amine) kit is designed for the efficient biotinylation of IgG molecules by immobilizing the IgG molecules on a solid support (see figure 1).   IgG molecules contain a histidine rich region in their Fc region and this fe..
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The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research.   A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail t..
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HyperCarrier™ is normal BSA that has been treated with ethylene diamine, which substitutes anionic carboxyl groups with cationic aminoethyl-amide groups.   Researchers were able to demonstrate that a cationized form of BSA, produced by replacing anionic side chain carboxylic groups with..
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G-Biosciences IgG Binding, Wash & Elution Buffers are designed for the non-denaturing, high yield purification of antibodies from IgG affinity purification resins.  The buffers are ideal for the purification of antibodies from  Protein A, Protein G and Protein A/G&n..
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Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge reason. Ficin is typically used to cleave mouse IgG1 as this are difficult to cleave with papain and pepsin. In the..
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Jacalin, or Artocarpus integrifolia lectin, is a tetrameric two-chain lectin with a molecular weight of 66kDa. Jacalin is a α-D-galactose binding lectin purified from jack-fruit (Artocarpus integrifolia) seeds.    Click here to request bulk or custom sizes.   Features ..
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Papain is a cysteine protease enzyme (EC 3.4.22.2) that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region.  This cleavage results in the generation of three ~50kDa fragments; two Fab domains and a Fc domain.  The papain-digested antibody is unable to p..
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Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)2 fragments from immunoglobulin G (IgG).  The pepsin has the ability to cleave the heavy chains near the hinge region. One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved..
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Immobilized Protein A/G consists of recombinant protein A/G ligand covalently immobilized onto 6% highly cross-linked agarose.  The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.   Protein A/G binds well to IgG subclasses but does ..
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Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). It is ideal for the easy one-step purification of classes, subclasses and fragments of immunoglobulins for biological fluids and cell culture media. Protein G is a modified form of Streptococca..
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