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Antibody Production & Purification

 

The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. The most common carrier proteins are bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH).

 

We offer a wide variety of carrier proteins for the successful generation of antibodies.Our ActiveHOOKproducts include both ActiveHOOK BSA (maleimide activated bovine serum albumin) and ActiveHOOKKLH (maleimide activated keyhole limpet hemocyanin). Both rapidly couple to free sulfhydryl groups on peptides and proteins using just one simple step. These activated carrier proteins save both time and money as separate cross-linkers are not required. Our HyperCarrier uses cationized BSA which has been found to elicit greater immunogenic response compared to normal BSA.

 

HOOK™  kits are also available for the coupling of peptides to carrier proteins. Both amine reactive and sulfhydryl reactive HOOKPeptide Coupling Kits are offered.

 

Download the Antibody Production Handbook for Complete Product Listing

Model:
Keyhole limpet hemocyanin is a widely used carrier protein due to its large molecular mass (4.5x105-1.3x107Da aggregates) and large number of primary amines that can react with cross-linkers for the coupling of peptides. The KLH is supplied as a clear to slightly hazy, bluish solution in phosphate b..
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Model:
Our No-Waste™ format adjuvant minimizes waste and risk of cross contamination.  Small dose packaging allows researchers to assign a vial per project or animal without concerns for excessive documentation and monitoring.  Researchers do not have the burden of storing large amounts of ..
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Our No-Waste™ format adjuvant minimizes waste and risk of cross contamination.  Small dose packaging allows researchers to assign a vial per project or animal without concerns for excessive documentation and monitoring.  Researchers do not have the burden of storing large amounts of ..
$0.00
The Pearl™ IgG Purification kits are designed for the one step purification of IgG (Immunoglobulin G) antibodies from serum. The supplied Pearl™ IgG Purification Resin binds the high abundant, non-IgG proteins (i.e albumin) and allows the IgG molecules to pass through in a physiolog..
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 Pearl™ IgG Purification Resin allows for the one-step purifiication of the  immunoglobulin G (IgG) antibodies from serum. The resin binds the high abundant, non-IgG proteins (i.e albumin) and allows the IgG molecules to pass through in a physiological buffer. The purified ..
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The Pearl™ Monoclonal IgG Purification kit allows for the rapid purification of antibodies from cell culture supernatant and ascites fluid. The Pearl™ IgG Purification Resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiol..
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Protein A is a cell-wall receptor which is present on cell wall of various starins of Staphyloccocus aureus. Protein A contains four high-affinity binding sites for interacting with the Fc region of immunoglobulin Gs of various species including mouse, rabbit and human. Protein A is used for making ..
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G-Biosciences' Protein G Magnetic Beads are Fe3O4 beads coated with dextran to produce highly uniform, 1µm beads. Recombinant Protein G is covalently coupled to the dextran coat to produce an enhanced alternative to agarose slurries for immunoprecipitation experiments. G-Biosciences m..
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The SDC™ (Steroid/ Drug/ Compound) Immobilization kit is designed for the immobilization of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification.  The ..
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The Sulfhydryl Immobilization Kit for Peptides is designed for the simple and efficient coupling of sulfhydryl-containing peptides to a solid agarose support. The Sulfhydryl Coupling Resin Columns utilizes iodoacetyl groups that specifically react with free sulfhydryls to form covalent, permanent th..
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The Sulfhydryl Immobilization Kit for Proteins is a complete kit designed for the simple and efficient coupling of proteins to a solid agarose support. The Sulfhydryl Coupling Resin Columns utilizes iodoacetyl groups that specifically react with free sulfhydryls to form covalent, permanent thioether..
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Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins.  Thiophilic adsorption was first developed by Porath et al1 in 1984 and is a group specific, salt-dependent purification technique that has distinct affinit..
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