CB-X™ Protein Assay (124 Citations)
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If the protein sample does not contain interfering agents, then a straightforward, single reagent assay is performed to give a linear response. If interfering agents are present or if artifactual results are produced, then the protein samples are treated with the clean up reagents and the protein is assayed to generate a linear response.
CB-X™ Protein Assay is supplied with lot-specific CB-X™ Tables. These allow researchers to perform single protein clean ups, subsequent assays, and then look up their absorbance in the CB-X™ Table to find the protein concentration. The CB-X™ Table eliminates the need for multiple protein standards and saves considerable time and effort. The table is prepared with a complex protein mixture that compares well with proteins from mammalian, plant, bacteria and yeast sources. The assay is supplied with a BSA protein standard or a non animal protein standard with Cat. # 786-12X or 786-894 respectively for generating curves when using CB-X™ Assay Dye alone or for researcher’s who prefer to generate their own standard curve or their own CB-X™ Table per their specific conditions.
DETERGENTS |
REDUCING AGENTS |
||
Brij® 35 |
2% |
2-mercaptoethanol |
1M |
CHAPS |
2% |
DTT |
1M |
CHAPSO |
2% |
CHAOTROPES |
|
Nonidet® P-40 |
2% |
Guanidine.HCl |
6M |
SDS |
2% |
Urea |
6M |
Triton® X-100 |
2% |
SALTS |
|
Tween® 20 |
2% |
Ammonium sulfate |
1M |
Deoxycholate |
0.1% |
MISCELLANEOUS |
|
SUGARS |
EDTA |
0.1M |
|
Glucose |
1M |
HEPES |
0.1M |
Sucrose |
25% |
MES |
0.1M |
Features
Applications
Protocol | |
786-12XT | ![]() |
786-11X | ![]() |
786-12X | ![]() |
786-894 | ![]() |
Material Safety Data Sheet | |
786-12XT | |
786-11X | |
786-12X | |
786-894 |
Technical Literature | |
A Non-Animal Alternative to BSA Protein Standards | |
Bioassays Handbook | |
Plant Proteomics Handbook | |
Protein Assay Handbook & Selection Guide | An introduction to protein assays. |
Protein Assay Handbook & Selection Guide | An introduction to protein assays. |