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Protein-S-S-Reductant™ (1 Citations)

A water soluble, odorless, non-toxic and stable protein reductant. Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pH's, compared to other reductants.
 
Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions.
 
Protein-S-S-Reductant™ is a ready-to-use solution that is at neutral pH and stabilized for long-term storage (1 year).
 
Simply supplement Protein-S-S-Reductant™ in place of DTT or ß-mercaptoethanol and boil the sample, before loading your gels.

 


Features

  • Ready-to-use solution, odorless, stable and non-toxic.
  • Neutral protein reduction solution.
  • Compatible with the alkylation reaction.
  • Works over a wide range of pH, including lower acidic pH's.

 


Applications

  • Reduction of protein disulfide bonds.
  • Reduction step for protein electrophoresis and other applications.
Protocol
786-25PR
Material Safety Data Sheet
786-25PR
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Technical Literature
Protein Cross-Linking Handbook  A detailed guide to protein cross-linkers and their reactions.
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Western Blotting Handbook From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.

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