2D-Detergent™ Nonidet® P-40 Substitute
Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and carbonyls. The peroxides will oxidize proteins and the carbonyl groups will form Schiff's bases with the proteins that interfere with a protein's structure.
Our sterile 2D-Detergent™ solutions contain reduced peroxides and carbonyl compounds (Figure). In addition, the detergents have less than 15µS conductivity. These detergents are offered as 10% aqueous solutions, sealed under inert gas and are suitable for all protein applications, including 2D-electrophoresis. This non-ionic detergent is suitable for isolating membrane-protein complexes.
The aldehyde and peroxide levels are <100µM and <10µM respectively with a conductivity of <15µS.
Low conductivity; <15µS
Low peroxide contamination.
Low carbonyl contamination.
- Reduced metal ions.
Sterile detergent solution
10% aqueous solutions.
Sealed under inert gas to prevent oxidation.
|Material Safety Data Sheet|
|Detergent Handbook & Selection Guide||A Comprehensive Guide to the Properties and Uses of Detergents in Research. Also features information on detergent removal systems.|
|Mass Spectrometry Sample Prep Handbook||A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.|