menu
Your Cart

Nonidet® P-40 Substitute (3 Citations)






Nonidet® P-40 Substitute

Nonidet® P-40 Substitute is a non-ionic detergent that is provided for research purposes or as a 10% proteomic grade solution.

Proteomic Grade: DG001, DG002, DG501, DG514


Research Grade: 786-511, 786-512

 


Proteomic Grade Detergent Solutions

Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds. The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and carbonyls. The peroxides will oxidize proteins and the carbonyl groups will form Schiff’s bases with the proteins that interfere with its structure.   

G-Biosciences’ Proteomic Grade Detergent Solutions contain reduced peroxides and carbonyl compounds. In addition, the detergents have less than 50µS conductivity. These detergents are offered  in a crimp style vial as sterile, 10% aqueous solutions, sealed under inert gas and are suitable for protein applications. These non-ionic detergents are suitable for isolating membrane-protein complexes.   

Other Proteomic Grade Detergent Solutions offered include: Triton® X-100Triton® X-114, Brij® 35Brij® 58Tween® 20 and Tween® 80. A variety pack is also available, perfect for trial and optimization. The pack contains one 10ml vial of all seven proteomic grade detergents, as well as one gram samples of one of our Zwitterionic detergents (CHAPS) and one of our non-detergent sulfobetaines (NDSB 201).

 


Features

  • Full Name: Nonylphenyl-polyethylene glycol
  • Molecular Formula: C15H24O[C2H4O]n
  • Molecular Weight: 573 (for n=8)
  • Absorbance (254nm): 0.14 (0.05% w/v)
  • Critical Micelle Concentration (CMC): ~0.05-0.3mM (25ºC)
  • Cloud Point: 76ºC

 


Applications

  • Non-ionic detergent for solubilizing membrane proteins during isolation of membrane-protein complexes
  • Ultra low concentrations of contaminating peroxides, aldehydes, salts and carbonyl compounds

 

 

Material Safety Data Sheet
786-511
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-512
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
DG001
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
DG002
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
DG501C
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
DG514C
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Assay Development A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.
Detergent Handbook & Selection Guide A Comprehensive Guide to the Properties and Uses of Detergents in Research. Also features information on detergent removal systems.
Removing Detergents from Protein Solutions
Certificate Of Analysis
786-511
182507

Write a review

Note: HTML is not translated!
Bad Good
Captcha

CONNECT WITH US