FOCUS™ Bacterial Proteome (4 Citations)
Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilizes nearly all of the proteins from E.coli, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilizes even the most difficult proteins. Our LongLife™ Lysozyme has improved stability and shelf life.
Simple extraction protocol.
Supplied with bacterial specific lytic enzyme preparation.
Supplied with a strong proprietary chaotropic extraction buffer.
Suitable for extraction from 50 x 50μl bacterial cell pellet.
Extraction of total proteins from bacteria.
Suitable for sample preparation for 2D gel electrophoresis and other applications.
|Material Safety Data Sheet|
|Mass Spectrometry Sample Prep Handbook||A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.|
|Plant Proteomics Handbook|
|Protease & Phosphatase Inhibitors & Proteases Handbook||A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays|
|Protein Electrophoresis Handbook||A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.|
|Sample Preparation Handbook||For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment|
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- Chen, H. et al (2012) Eur. J. Pharmacol. 690:51
- Darby, AC et al (2012) Genome Res. 22:2467
- Rigobello, M.P. et al (2008) Eur. J. Pharmacol. 582:26