FOCUS™ Signal Proteins (20 Citations)
Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signaling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localized proteins have been shown to be involved in intracellular membrane trafficking and signaling.
100mg mammalian tissue
50µl wet animal cell pellet
50µl wet yeast pellet
50µl wet bacteria pellet
250mg plant tissue
Fractionate lipid raft localized proteins.
A 2D electrophoresis compatible buffer is provided.
Isolate signal proteins that are localized to lipid rafts.
Study movement of activated proteins to and from lipid rafts.
Suitable for 2D gel electrophoresis, proteomics and cell signaling studies.
|Material Safety Data Sheet
|Mass Spectrometry Sample Prep Handbook
|A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
|Protein Electrophoresis Handbook
|A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
|Sample Preparation Handbook
|For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment