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FOCUS™ Mitochondria (14 Citations)

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Specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria.  The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality. 

This kit is highly adaptable for use with animal tissues and other sources of mitochondria. The kit is designed for 50 preps, where one prep is equivalent to 2x107 mammalian cells or 100mg mammalian tissue.

 


Features

  • Fast and efficient fractionation of active heavy & light mitochondria
  • Suitable for cultured animal cells and tissues

 

Applications

  • Fraction of mammalian cells and tissues into a mitochondrial rich fraction
  • Suitable for sample preparation for 2D gel electrophoresis and other applications
Protocol
786-022
Material Safety Data Sheet
786-022
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Technical Literature
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Sample Preparation Handbook For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment

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