G-Trap™ HIC Selection Kit consists of 4 prepacked 1 ml columns (G-Trap™ HIC Columns) with matrix or resin for hydrophobic interaction chromatography. The kit consists of 4 pepacked columns namely G-Trap™ Butyl Agarose 6 Fast Flow, G-Trap™ Octyl Agarose 6 Fast Flow, G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) and G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub).
G-Trap™ HIC Selection Kit allows for initial screening of appropriate ligand/matrix and conditions for isolation of a specific protein or biomolecule. Once appropriate resin/medium is determined, individual G-Trap™ HIC Column in 1 ml or 5 ml sizes can be used. Individual G-Trap™ HIC Columns are available at G-biosciences in 1 ml and 5 ml column volume size
The G-Trap™ HIC Columns available for seperataion of the target protein includes G-Trap™ Butyl Agarose 6 Fast Flow, G-Trap™ Octyl Agarose 6 Fast Flow, G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) and G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub).
Biomolecules are separated on the HIC column based upon the hydrophobicity of the ligand attached to the resin, the distribution of surface-exposed hydrophobic amino acids present on the protein or enzyme to be separated and the concentration and type of salt used in binding or start buffer.
Straight alkyl chains ligands such as butyl, octyl show pure hydrophobic character whereas aryl ligands such as phenyl show mixed mode behavior where aromatic, hydrophobic as well as lack of charge play role in protein/biomolecules adsorption to the column. In HIC, the binding of biomolecules to the column (ligand) is facilitated by using higher concentrations of anti-chaotropic salts such as ammonium sulfate, sodium sulphate etc in the binding buffer.
The properties of G-Trap™ HIC Selection Kit resin and column are listed below
G-Trap™ HIC Selection Kit resin
G-Trap™ Butyl Agarose 6 Fast Flow Resin
Mean particle size: 90 µm
Bead structure: Highly cross-linked 6% Agarose
Ligand: Butyl
Ligand Concentration: About 40μmol/ml
Binding Capacity: About 20mg HSA/ml resin
pH stability Working Range: 3-13
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Pressure (MPa): 0.3
Maximum Flow Velocity: 450cm/h
Exclusion Limit (Globular Proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol
G-Trap™ Octyl Agarose 6 Fast Flow Resin
Mean particle size: 90 µm
Bead structure: Highly cross-linked 6% Agarose
Ligand: Octyl
Ligand Concentration: About 5μmol/ml
Binding Capacity: About 30mg HSA/ml resin
pH stability Working Range: 3-13
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Pressure (MPa): 0.3
Maximum Flow Velocity: 450cm/h
Exclusion Limit (Globular Proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol
G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) Resin
Mean particle size: 90 µm
Bead structure: Highly cross-linked 6% Agarose
Ligand: Phenyl
Ligand Concentration: About 40μmol/ml
Binding Capacity: About 40mg HSA/ml resin
pH stability Working Range: 3-13
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Pressure (MPa): 0.3
Maximum Flow Velocity: 450cm/h
Exclusion Limit (Globular Proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol
G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub) Resin
Mean particle size: 90 µm
Bead structure: Highly cross-linked 6% Agarose
Ligand: Phenyl
Ligand Concentration: About 25 μmol/ml
Binding Capacity: About 20 mg HSA/ml resin
pH stability Working Range: 3-13
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Pressure (MPa): 0.3
Maximum Flow Velocity: 450 cm/h
Exclusion Limit (Globular Proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol
G-Trap™ HIC Selection Kit, 1 ml column
Column Volume: 1 ml
Columns Dimension: 0.7 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)
Application(s):
- G-Trap™ HIC Selection Kit allows for initial screening of appropriate ligand/matrix and conditions for isolation of a specific protein or biomolecule. The kit consists of 4 pepacked columns namely G-Trap™ Butyl Agarose 6 Fast Flow, G-Trap™ Octyl Agarose 6 Fast Flow, G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) and G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub).
