Periodic Acid Schiff staining technique is used to demonstrate simple polysaccharides, neutral muco-polysaccharides, mucoproteins, and glycolipids. In this kit, Mayer’s hematoxylin is used to stain cell nuclei.
Uses and sample preparation:
Designed for research use only, it is not approved for human or animal use, or clinical diagnosis.
Components
Short Procedure
- Deparaffinize and hydrate
- Add periodic acid 0,5%
- Rinse with distilled water
- Add Schiff Reagent
- Rinse with 3 baths of sulfurous acid
- Rinse with tap water
- Place in hematoxylin
- Rinse with tap water
- Dehydrate, clear and coverslip
Staining characteristics:
Nuclei can be visualized in violet, simple polysaccharides, neutral muco-polysaccharides, glycolipids and basement membrane in red/purple
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