menu
Your Cart

HOOK™-NHS-Biotin Labeling Kit

HOOK™ NHS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35 are available on the surface of the molecules and can be reacted with amine reactive esters.

 

The kit is designed for the conjugation of biotin to protein primary amine groups and is supplied with 25mg HOOK™ NHS-Biotin, a specific Optimizer Buffer™, for enhanced conjugation, Spin-OUT™ columns, for purification of labeled protein, and reagents to determine the amount of biotinylation. 

 

Each kit is designed for the coupling of 1-10mg protein in 1ml buffer, suitable for 10 couplings.
 

 

This product is an improvement of BK-01 and its direct replacement.

 


Features

  • Synonym: N-Succinimidyl D-biotinate, succinimidyl biotin
  • CAS Number: 35013-72-0
  • Linear Formula: C14H19N3O5S
  • Molecular Weight: 341.38
  • Spacer Arm (Å): 13.5
  • Reactive Group: NHS-ester
  • Membrane Permeable: YES
  • Water Soluble: NO
  • Cleavable/ Reversible: NO
  • Reaction pH: 7-9 

 


Applications

  • Complete kit for labeling protein primary amines
  • Labels 1-10mg protein/reaction
  • Suitable for 10 coupling reactions

 

 

Protocol
BS-01
Material Safety Data Sheet
BS-01
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Antibody Handbook
Assay Development A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.
NHS Ester Reactivity Assay
Protein Labeling & Conjugation

Write a review

Note: HTML is not translated!
Bad Good
Captcha

CONNECT WITH US