Phycobiliproteins are fluorescent proteins obtained from cyanobacteria and eukaryotic algae. The fluorescence of these proteins is very high, when compared to chemical fluorescent probes, such as fluorescein and rhodamine. R-Phycoerythrin (R-PE) is a phycobiliprotein derived from Porphyra tenera, an algae. It has bright red fluorescence at around 578 nm. It is excited from 488-565nm with absorption maximum at 496, 546 and 565 nm.
R-Phycoerythrin (R-PE) labelled antibodies and other molecules can provide greater sensitivity in immunostaining and flowcytometry compared to chemical fluorescent probes.
G-Biosciences HOOK™ Maleimide Activated R-Phycoerythrin is offered to enable its conjugation with antibodies or other molecules for its application in immunostaining and flowcytometry. R-Phycoerythrin is maleimide activated using Sulfo-SMCC, a heterobifunctional crosslinker, which adds a free maleimide group, which reacts with sulfhydryl containing molecules.
Features:
- Sulfo SMCC activated R-Phycoerythrin
- Sulfhydryl Reactive: Conjugates with molecules containing reduced thiol (-SH) groups.
- Available as ready to use 1 mg lyophilized vial.
Application(s):
- HOOK™ Maleimide Activated R-Phycoerythrin conjugates with antibodies for high sensitive immunostaining and flowcytometry applications.
