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Protein A/G Complete Antibody Clean Up

Protein-AG-Complete-Antibody-Clean-Up

Protein A/G Complete Antibody Clean Up is designed for complete removal of stabilizers such as BSA, gelatin and the amine buffers that interfere with downstream application such as labeling and conjugation. The kit also purifies IgGs from serum, plasma and ascites.


Protein A/G Complete Antibody Clean Up contains Protein A/G Resin which is recombinant protein A/G ligand covalently immobilized onto 4% highly cross-linked agarose.   Protein A/G has high binding capacity of 38 mg human IgG/ml resin and >20 mg sheep IgG/ml resin.

 

Protein A/G Complete Antibody Clean Up kit contains reagents suitable to carry out 20 small scale purifications to purify 100 µg antibodies per purification. To purify >100 µg antibodies increase the Protein A/G Resin amount proportionally.

 

Features

  • Removes stabilizers such as BSA and gelatin completely from antibody.
  • Removes amine buffers as purification of antibody is carried out in amine free buffers.
  • Suitable for conjugation and labeling reactions.
  • Kit contains reagents to carry out 20, 100µg antibodies purification.

 

Applications

  • Complete removal of stabilizers such as BSA, gelatin and the amine buffers that interfere with downstream application such as labeling and conjugation.
  • The kit also purifies IgGs from serum, plasma and ascites for downstream applications.

 

Protocol
786-1604

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