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Botanical Garden Blot

The blot is prepared using total protein extracted from a variety of 5-10 day old, whole plant tissues. 

 

The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker.  After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer Buffer.

 

The blots are ready to be blocked and probed with the antibodies of choice.

 

Note: We recommend that customers contact our technical department for the latest information on the blots as G-Biosciences reserves the right to change the blot profile due to the availability of GenLysate.

 


Features

Blot lane order:

  1. Protein marker
  2. Green Algae Lysate
  3. Yeast Lysate
  4. Barley Lysate
  5. Sweet Corn Lysate
  6. Rye Grass Lysate
  7. Oat Lysate
  8. Wheat Lysate
  9. Soy Lysate
  10. Mung Lysate
  11. Sunflower Lysate
  12. Cotton Lysate
  13. Beet Lysate
  14. Tomato Lysate
  15. Spinach Lysate

 

Material Safety Data Sheet
BB01
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