The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer Buffer.
The blots are ready to be blocked and probed with the antibodies of choice.
Note: We recommend that customers contact our technical department for the latest information on the blots as G-Biosciences reserves the right to change the blot profile due to the availability of GenLysate.
Features
Blot lane order:
- Protein marker
- Tumor Ovary Lysate
- Tumor Lung Lysate
- Tumor Liver Lysate
- Tumor Rectum Lysate
- Tumor Cervix Lysate
- Tumor Skin Lysate
- Tumor Testis Lysate
- Tumor Thyroid Lysate
- Tumor Uterus Lysate
- Tumor Stomach Lysate
- Tumor Hypopharynx Lysate
- Tumor Breast Lysate
- Tumor Prostate Lysate
- Tumor Pancreas Lysate
English_US