Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins, and suppress peptide ionization when analyzed by mass spectrometry.
DetergentOUT™ GBS10 features a research grade detergent binding resin that has a high affinity for most detergents and a low affinity for proteins and peptides. DetergentOUT™ GBS10 is ideal for detergent removal prior to ELISA, IEF, protease digestion of proteins, and peptide ionization when analyzed by mass spectrometry.
DetergentOUT™ GBS10 is available in four spin format column sizes or as resin alone. The column specifications are:
| Cat. # |
Description |
Sample Size (µl) |
Resin Volume (µl) |
| 786-154 |
DetergentOUT™ GBS-10-125 |
10-30 |
125 |
| 786-155 |
DetergentOUT™ GBS-10-800 |
30-200 |
800 |
| 786-156 |
DetergentOUT™ GBS-10-3000 |
200-750 |
3,000 |
| 786-157 |
DetergentOUT™ GBS-10-5000 |
500-1,250 |
5,000 |
Our DetergentOUT™ GBS10 resin removes free, unbound anionic, nonionic or zwitterionic detergents (e.g. SDS, Triton® X-100 or CHAPS) from aqueous protein and peptide samples with minimal sample loss (Table below) for downstream analysis by mass spectrometry (Figure 1 & 2) and other techniques.
Also available in 96-well spin plate formats for processing up to 96 samples.
The DetergentOUT™ GBS10 columns were shown in independent studies to be fully compatible with DI-QTOF and LC-MS/MS (see references). The use of the DetergentOUT™ GBS10 columns significantly increased the number of peptide spectra detected (Figure 1 & 2).
In addition, the DetergentOUT™ GBS10 columns have a high binding capacity for detergents, with 6 milligrams SDS (Figure 3) and 14 milligrams Triton® X-100 for every milliliter of settled resin.
| Detergent |
% Removed |
BSA |
Phosphorylase B |
Cytochrome C |
E.coli Lysate |
| Triton X-100, 2% |
>99 |
>90 |
>91 |
>92 |
>93 |
| Triton X-114, 2% |
>96 |
>99 |
>98 |
>97 |
>91 |
| Nonidet P-40, 1% |
>96 |
>93 |
>95 |
>91 |
>91 |
| Brij 35, 1% |
>99 |
>98 |
>99 |
>97 |
>91 |
| SDS, 2.5% |
>99 |
>96 |
>97 |
>92 |
>90 |
| Sodium deoxycholare, 5% |
>99 |
>99 |
>99 |
>98 |
>95 |
| CHAPS, 3% |
>99 |
>92 |
>95 |
>92 |
>91 |
| Octyl glucoside, 5% |
>99 |
>93 |
>95 |
>96 |
>91 |
| Lauryl maltoside, 1% |
>97 |
>99 |
>99 |
>99 |
>91 |
| Tween 20*, 0.25% |
>98 |
>86 |
>85 |
>89 |
>85 |
| Tween 80*, 0.13% |
>85 |
>83 |
>81 |
>80 |
>81 |
Detergent removal rates and percentage protein recovery with DetergentOUT™ GBS10
Features
- Easy-to-use, spin-format columns
- Rapid removal of free detergents
- Suitable for anionic, non-ionic and zwitterionic detergents
- Minimal sample loss
- Available for sample volumes ranging from 10µl to 1,250µl
Applications
- Detergent removal from protein and peptide solutions
- Detection of peptide fragments by Mass spectrometry
- Enhances Mass spectrometry Spectra
- Ideal for Mass spectrometry sample preparation and prior to downstream analysis and applications
