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Nickel Chelating Resin









Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni2+) for the purification of 6x histidine tagged proteins.

 

This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked agarose beads. The iminodiacetate binds divalent nickel ion with a capacity of 20-40μmoles Ni2+/ml resin.  The protein binding capacity is >50mg protein per ml resin.  We have demonstrated binding of >100mg of a 50kDa 6XHis tagged proteins to a ml of resin. A Nickel NTA resin is also offered.

 

The spin columns are supplied with a resin bed volume of 0.2, 1 and 3ml with total column volumes of 1, 8 and 22ml respectively. Columns can be used as a spin format of gravity flow columns.

 

Immobilized Copper, Cobalt  and Zinc  Chelating Resins are also available.  Cobalt has the highest selectivity followed by Zinc, Nickel then Copper, but has the lowest binding capacity.  Copper has the highest binding capacity, followed by Nickel then Zinc.

 

Specific binding/wash and elution buffers are available.

 

Our new HOOK™ 6X His Protein Purification kits are now available and include everything required for purification of 6X His tagged proteins from yeast or bacteria, including lysis buffers, lytic enzymes, resin, columns and binding, wash and elution buffers. Each kit is available with nickel or cobalt chelating resins. Kits available are:
HOOK™ 6X His Protein Purification (Bacteria): Isolation from bacteria; optimized to yield up to 10mg/250ml culture of soluble 6X His tagged protein.
HOOK™ 6X His Protein Spin Purification (Bacteria): Isolation from bacteria;optimized to yield ~1mg/50ml culture of soluble 6X His tagged protein
HOOK™ 6X His Protein Purification (Yeast): Isolation from yeast; optimized to yield up to 10mg of soluble 6X His tagged protein.
HOOK™ 6X His Protein Spin Purification (Yeast): Isolation from yeast; optimized to yield up to 1mg of soluble 6X His tagged protein.
 
 

 

 


Features

  • For the purification of 6x His proteins
  • High capacity: >50mg/ml
  • Ligand density: 20-40μmoles Ni2+ /ml resin
  • Bead Structure: 6% cross-linked agarose

 



Applications

  • Affinity purification of proteins with a 6x His tag.

 

 

Protocol
786-281
786-392
786-393
786-394
786-407
786-408
786-429
SP-786-407
SP-786-408
Material Safety Data Sheet
786-281
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786-392
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786-393
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786-394
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786-407
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786-408
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786-429
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Dutch
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German
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Portuguese
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SP-786-407
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
SP-786-408
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Plant Proteomics Handbook
Protein Purification Handbook

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