Immobilized Papain (3 Citations)
Papain is a cysteine protease enzyme (EC 22.214.171.124) that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. This cleavage results in the generation of three ~50kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonization, and lysis.
The Fab and Fc fragments can be further separated with either Immobilized Protein A or ion exchange chromatography.
Immobilized Papain is a convenient reagent for producing Fab and Fc fragments as it avoids the need to remove the papain enzyme after digestion.
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- Generate Fc and Fab from IgG
- Eliminates contamination with papain enzyme
- Can be used in virtually all scenarios using free papain
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|Protease & Phosphatase Inhibitors & Proteases Handbook||A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays|
- Anand, S. O. et al (2019) Antibody-induced internalization of HIV-1 Env proteins limits the surface expression of the closed conformation of Env.J. Virol.DOI: 10.1128/JVI.00293-19.
- Tolbert, W. D. et al (2016) Paring Down HIV Env: Design and Crystal Structure of a Stabilized Inner Domain of HIV-1 gp120 Displaying a Major ADCC Target of the A32 Region.Journal/ Year/ Vol #: Structure/2016/24
- Zhang, H. amd Lin, S. (2003) J. Phycol. 39:1160