Well-Coated™ Biotin

Well-Coated™ Biotin plates are designed to specifically bind avidin, streptavidin or Neutravidin™ conjugated molecules, including enzyme conjugates. 

Biotin exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵M^-1) and streptavidin (Ka=10¹⁵ M^-1).  Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH1.5 or by autoclaving.  Streptavidin and Neutravidin™ in many respects are similar to avidin except that they have  no carbohydrate and their solubility in aqueous buffer is much lower than avidin.  Neutravidin™ also lacks the RYD sequence eliminating interaction with RGD domain of adhesion receptors.   The binding of strepatavidin and Neutravidin™ is similar to that of avidin, but with less non-specific binding. 

The wells are coated to a 200µl depth and are supplied pre-blocked in our proprietary Superior™ Blocking Buffer.  The plates have the capacity to bind 30-50ng streptavidin per well.  

The clear, white and black plates are offered for colorimetric, chemiluminescence and fluorescent detection systems, respectively.
 

Features

• Binds avidin, streptavidin and Neutravidin™ conjugated molecules
• Reduced non-specific binding as plates are pre-blocked with Superior™ Blocking Buffer