Well-Coated™ Protein L plates are designed to bind the kappa light chains of immunoglobulins without interfering with the antigen binding site. The Well-Coated™ Protein L plates offer greater range of immunoglobulin classes and subclasses compared to Protein A, Protein G and Protein A/G. Protein L will bind to all classes of IgG, including IgG, IgM, IgA, IgE and IgD, and binds to single chain variable fragments (scFv and Fab fragments).
Well-Coated™ Protein L plates are for single antibody assays and are not suitable for multiple assays (sandwich ELISAs) as the first antibody will not block all IgG binding sites and therefore false positives will occur with the second antibody. The wells are coated to a 100µl depth and are supplied pre-blocked in our proprietary Superior™ Blocking Buffer. The clear, white and black plates are offered for colorimetric, chemiluminescence and fluorescent detection systems, respectively.
Features
- Retains antibody activity
- Binds to all classes of IgG, including IgG, IgM, IgA, IgE and IgD
- Reduced non-specific binding as plates are pre-blocked with Superior™ Blocking Buffer
