Protein-Free Blocking Buffer (2 Citations)
Protein-Free Blocking Buffer does not contain protein; it is a proprietary formation of non-protein agents that eliminates non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free Blocking Buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.
Protein-Free Blocking Buffer eliminates any concern associated with regulatory compliance issues where use of animal source components are restricted. Furthermore, Protein-Free™ Blocking Buffer is compatible with antibodies and avidin/biotin based systems and results in high signal to background ratios.
For user’s convenience Non-Protein Blocking Buffers are supplied in widely used TBS (Tris-buffered saline at pH 7.5) and PBS buffers (phosphate-buffered saline at pH 7.5) as well as in separate formulations containing Tween®-20 for improving blocking efficiencies.
- Protein free blocking agent
- Eliminate or minimize cross reactivity with animal source antibodies and antigens
- High signal to background ratios
- Four convenient formats, with and without detergent
|Material Safety Data Sheet|
|Assay Development||A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.|
|Western Blotting Handbook||From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.|
|Certificate Of Analysis|
- "Gao, Steven et al (2022) Nanocell COVID-19 vaccine elicits iNKT-licensed dendritic cells to produce high affinity antibodies neutralizing Variants of Concern. RESEARCH SQUARE. https://orcid.org/0000-0002-4969-1151
- Tsai, T. F. et al (2019) Benzyl isothiocyanate promotes miR‐99a expression through ERK/AP‐1‐dependent pathway in bladder cancer cells. Environ. Toxicol. doi.org/10.1002/tox.22841.