Swift™ Western Blotting System (1 Citations)
The Swift™ Western Blotting System is a unique system that reduces the blocking and antibody incubations on Western blot membranes from >4 hours to <60 minutes.
Using a combination of proprietary wash and diluent buffers and our highly sensitive femtoLUCENT™ chemiluminescence detection reagent the Swift™ Western Blotting System generates comparable result to traditional Western blotting procedures and other commercial “fast” Western blotting kits.
An added advantage is that Swift™ Western Blotting System is designed to be used with any combination of primary and secondary antibodies, unlike other commercial kits that limit researcher’s to rabbit or mouse primary antibodies.
Swift™ Western Blotting System is compatible with wet and semi-dry transfer systems and with all automated transfer systems on the market. The system is suitable for 8 blots (8 x 10cm); the sample size is suitable for 2 blots.
Features
- Sensitive: Femotgram level sensitivity
- Fast: Reduce blot development to <60 mins
- Versatile: Compatible with all combnations of primary and secondary antibodies
- For all wet, semi-dry and automated blotting systems
Applications
- Rapidly develop blots in <60 minutes compared to > four hours for standard blotting protocols
Protocol | |
786-158 |
Material Safety Data Sheet | |
786-158 |
Technical Literature | |
Plant Proteomics Handbook | |
SWIFTâ„¢ Western Diluent | SWIFT™ Western Diluent is a new generation Western blotting reagent. The single reagent SWIFT™ Western Diluent simplifies protein detection by Western blotting and reduces the overall time spent on Western blot development. SWIFT™ Western Diluent has been developed to eliminate the time consuming blocking step Eliminate Time Consumming Blocking Step Minimal Optimization Compatible with All Antibody Combinations Non Denaturing The application note shows how SWIFT™ Western Diluent shortens Western blotting with no effect on sensitivity Download the free application note to find the key to faster, affordable Western blots! |
Western Blotting Handbook | From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins. |