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femtoLUCENT™ PLUS-HRP

Catalog Description Size Price(USD) Qty
786-003
femtoLUCENT™ PLUS-HRP Chemiluminescent reagents only 100ml
$162.00
786-056
femtoLUCENT™ PLUS-HRP Chemiluminescent reagents only 200ml
$316.00
786-081
femtoLUCENT™ PLUS-HRP Chemiluminescent reagents only 500ml
$774.00
786-10
femtoLUCENT™ PLUS HRP Kit 100ml kit
$216.00
786-10T
femtoLUCENT™ PLUS HRP Chemiluminescent reagents only 20ml
$92.00

femtoLUCENT™ PLUS-HRP

femtoLUCENT™ PLUS is a femtogram level sensitive chemiluminescence immunodetection system that allows users the option to detect even hard-to-detect low abundance proteins. This immunodetection system is based on an innovative formulation of luminol and 1, 2 dioxetane, a supersensitive detection reagent for HRP (horseradish peroxidase).  The chemiluminescence light emission can be recorded by a short exposure to autoradiography films. 

 

femtoLUCENT™ PLUS-HRP reagents are available in three sizes suitable for 5, 25, 50 and 125 blots as each 4ml of working solution is suitable for 1 mini blot (8 x 7.5cm)

 

In addition, femtoLUCENT™ PLUS-HRP is also supplied in a kit format, containing our non-animal protein blocking agent (NAP BLOCKER™) and  wash buffer (femto-TBST™).  The femtoLUCENT™ PLUS-HRP kits allow detection of low femtogram levels (10-15) of antigens with low noise (signal/background) ratio.  The kit reagents are sufficient for 25 mini blots or 1,500cm2 of PVDF or nitrocellulose membrane.

 

For a complete chemiluminescence immunodetection system, we offer optional alkaline phosphatase (AP) conjugated secondary antibodies against mouse, rabbit, human, rat and goat. 

 


Features

  • Reagents alone or kit formats
  • Intense light emission with low background and high signal
  • Supplied with a novel blocking agent to allow a rapid blocking step that produces a clear background
  • Economical: greater value compared to similar products

 


Applications

  • Quick and sensitive non-isotopic detection of proteins on transfer membranes (suitable for nitrocellulose & PVDF membranes)
  • Immunodetection of proteins and antigens for Western blots and dot blot applications
  • Low femtogram detection (10-15)that allows for detection of >10fg protein on a dot blot and >1pg on a Western blot
Protocol
786-003
786-056
786-081
786-10
786-10T
Material Safety Data Sheet
786-003
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
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786-056
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-081
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-10
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-10T
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
Technical Literature
Western Blotting Handbook From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.
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