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PBST [10X]

Catalog Description Size Price(USD) Qty
R044
PBST [10X]; Phosphate buffered saline with Tween-20 1L
$63.00
R045
PBST [10X]; Phosphate buffered saline with Tween-20 1 Gallon
$187.00

PBST [10X]

A 10X Concentrated solution of Phosphate Buffered Saline with Tween® 20. The 10X Concentration is 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween® 20, pH 7.4.

 

PBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. The Phosphate Buffered Saline with Tween® 20 is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from membranes and microtiter plate wells without disrupting the antigen/antibody binding reaction.

 

By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.

 

Features

  • 1X Concentration is 8mM Na2HPO4, 150mM NaCl, 2mM KH2PO4, 3mM KCl, 0.05% Tween® 20, pH 7.4

 

Applications

  • Wash buffer for Western Blotting and ELISA
Material Safety Data Sheet
R044
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R045
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
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