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Zymogram Renature Buffer [10X]

Catalog Description Size Price(USD) Qty
786-482
Zymogram Renature Buffer [10X] 125ml
$21.00

Zymogram Renature Buffer [10X]

Zymography is an electrophoretic technique, based on SDS-PAGE, that includes an enzyme substrate copolymerized with the polyacrylamide gel.  Samples are prepared in Zymogram Sample Buffer without boiling to preserve the structure and activity of the enzyme. Following electrophoresis, the SDS is removed from the gel by washing in Zymogram Renature Buffer that contains a non-ionic detergent. The gels are then equilibrated in Zymogram Developing Buffer, which contains the divalent metal cation required for enzymatic activity.  The zymogram is subsequently stained (commonly with Amido Black or Coomassie Brilliant Blue), and areas of digestion appear as clear bands against a darkly stained background where the substrate has been degraded by the enzyme.

 


•    Zymogram Sample Buffer [2X]: 62.5mM Tris, 4% SDS, 25% Glycerol, 0.01% Bromophenol Blue, pH 6.8


•    Zymogram Renature Buffer [10X]: 25% Triton® X-100


•    Zymogram Development Buffer [10X]: 0.5M Tris, 2M NaCl, 50mM CaCl2, 0.2% Brij® 35, pH 7.5
 

 


Features

  • Zymogram Renature Buffer [10X]: 25% Triton® X-100

 

 

Material Safety Data Sheet
786-482
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Technical Literature
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
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