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An improved Coomassie Dye based protein assay based on the Bradford Protein Assay (1). This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in a change of color from brown to blue. The change in color density is proportional to protein concentration. Protein estimation can be performed using as little as 0.5µg protein
CB™ Protein Assay is supplied with a simple to follow protocol. Ready to use reagent that does not require prefiltering or dilution. Simply mix the protein solution with CB™ Protein Dye and read optical density.
Compound | Concentration | Compound | Concentration | Compound | Concentration |
Amino acids | 1mM | DTT | 1M | Phenol | 5% |
Ammonium sulfate | 1M | EDTA | 100mM | Sodium azide | 0.5% |
Ampholytes | 0.5% | EGTA | 50mM | Sodium chloride | 6M |
Ascorbic acid | 50mM | Ethanol | 10% | Sodium dodecyl sulfate (SDS) | 0.015% |
Boric acid | 1mM | Glucose | 1M | Sodium hydroxide | 0.1M |
Brij® 35 | 0.06% | Glycerol | 10% | Sodium phosphate | 0.1M |
CHAPS | 0.5% | Glycine | 0.1M | Sucrose | 25% |
CHAPSO | 0.5% | Guanidine.HCl | 6M | Tris | 2M |
Citrate | 0.05% | HEPES | 0.1M | Triton® X-100 | 0.06% |
Cysteine | 10mM | 2-mercaptoethanol | 1M | tRNA | 0.35mg/ml |
Deoxycholate | 0.1% | Methanol | 10% | Tween® 20 | 0.03% |
DMSO | 10% | MES | 0.7M | Urea | 3M |
DNA | 1mg/ml | Nonidet® P-40 | 0.5% |
Features
Applications
Protocol | |
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Material Safety Data Sheet | |
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786-012T | |
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Technical Literature | |
A Non-Animal Alternative to BSA Protein Standards | |
Bioassays Handbook | |
Protein Assay Handbook & Selection Guide | An introduction to protein assays. |
Certificate Of Analysis | |
786-012 |