Amine Magnetic Beads
G-Biosciences’ Amine Magnetic Beads are 1µm, uniform magnetic beads with an amine (-NH2) surface functional group. The magnetic beads consist of a single-crystal Fe3O4 sphere core and dextran coating layer. Through chemical modification of dextran, the primary amino group (-NH2) are joined to the magnetic beads through a short hydrophilic linker. The hydrophilic surface ensures the magnetic beads excellent dispersion ability and easy handling property in a wide variety of buffers.
The magnetic beads with surface-reactive amino groups allow immobilization of ligands such as proteins, peptides, carbohydrates or other target specific molecules. Immobilization of ligands can be through reductive amination of aldehyde or ketones without prior activation of the bead surface. Alternatively, carbodiimide cross-linkers can be used to couple ligands to the amines via their carboxyl groups. Finally, amine reactive heterobifunctional cross-linkers can be used to introduce other functional groups for coupling ligands.
Carbodiimide activation of carboxyl groups produces a very labile intermediate that hydrolyzes quickly, meaning the ligand needs to be added rapidly. Alternatively, a two step protocol using N-hydroxysuccinimide (NHS) can be employed to produce a less labile intermediate that reacts over a longer time period.
- 1µm beads
- ~50mM ligand density
- Rapid binding at neutral to high pH
- Compatible with a large range of amine reactive cross-linking reagents
- No centrifugations required
- Dextran coating for low non-specific binding
- Isolation of proteins and peptides
- C-terminal coupling of peptides