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G-Sep™ Ni IDA Agarose Fast Flow

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  G-Sep™ Ni IDA Agarose Fast Flow is specifically designed for the purification of recombinant proteins fused to the 6x histidine (6XHis) tag.
 
The resin is  specifically designed for the purification of recombinant proteins fused to the 6x histidine (6XHis) tag expressed in bacteria, insects, and mammalian cells.  The resin is high affinity and selectivity for recombinant fusion proteins that are tagged with six tandem histidine residues.  
 
G-Sep™ Ni IDA Agarose Fast Flow (FF) resin is nickel ions immobilized onto highly cross-linked 6% agarose beads using iminodiacetic acid groups (IDA). The G-Sep™ IDA Agarose Fast Flow (FF) resins have high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. G-Sep™ Co IDA Agarose Fast Flow (FF) resin using cobalt is also available
 
 

Features
  • Matrix: Cross-linked agarose beads, 6%
  • Bead form: Spherical, diameter 50-160μm
  • Spacer: Epichlorohydrin
  • Chelating Agent: Iminodiacetic acid
  • Active group: Ni2+
  • Ni2+ density: 20-40μmol /ml
  • Binding Capacity: 5-10mg His-tagged protein/ml medium
  • pH stability Working Range: 3-12
  • pH stability Cleaning-in-Place (CIP): 2-14
  • Maximum Flow Velocity: 450cm/h
  • Exclusion limit(globular proteins): 4 x 106
  • Physical Stability: Negligible volume variation due to changes in pH or ionic strength
  • Chemical Stability: Stable to all commonly used aqueous buffers: 6M urea, 8M guanidine hydrochloride,
  • Autoclavable: 121°C, pH 7, for 30 min
  • Storage Conditions: 2 to 8°C, 20% Ethanol

 


Applications

  • Immobilized meatl affinity chromatography (IMAC)
  • Purification of 6X His tagged proteins
  • Purification of nickel binding proteins

 

 

Protocol
786-973
786-974
786-975
786-976
Material Safety Data Sheet
786-973
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786-974
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-975
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-976
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish

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G-Sep™ Ni IDA Agarose Fast Flow
G-Sep™ Ni IDA Agarose Fast Flow
Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines,..
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