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Histidine-Tagged Protein Purification

G-Biosciences provide a range of Immobilized Metal Affinity Chromatography (IMAC) resins for 6X His tagged protein purification.

Four  IMAC purification resins are offered:

Nickel Chelating ResinThe most commonly used IMAC purification resin for the purification of 6X His recombinant proteins that offers high binding efficiency and low non-specific binding. 

Cobalt Chelating ResinGrowing increasingly popular due to its advantage over Nickel Chelating Resin.  Although 6X His recombinant proteins bind with a slightly lower efficiency compared to Nickel Chelating Resin there is a significant reduction in non-specific binding. Cobalt resins have a higher selectivity for poly-His sequences, however have a low loading capacity, therefore Cobalt Chelating Resin should be used for valuable recombinant proteins in limited quantities. 

Nickel and Cobalt chelating resins are available as a resin slurry or 0.2mL, 1mL, and 3mL spin columns.

Zinc Chelating ResinFor the purification on zinc binding proteins, including 6x His recombinant proteins. Available as a resin.

Copper Chelating ResinFor the purification on copper binding proteins, including 6x His recombinant proteins. Available as a resin.

Cobalt has the highest selectivity of the resins followed by Zinc, Nickel then Copper, but has the lowest loading capacity. Copper has the highest loading capacity, followed by Nickel then Zinc.

Download the Protein Purification Handbook for a full list of related productsDownload the Purification Resins Handbook for Complete Product List

HOOK™ 6X His Protein Spin Purification kit allows for the rapid purification of soluble, 6X His tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and a..
G-Biosciences’ Ni-NTA Magnetic Beads are 3µm beads designed for the rapid purification of X His-tagged proteins. Ni-NTA Magnetic Beads have nitrilotriacetic acid (NTA) groups with charged nickel covalently bound to the surface dextran of the beads.  Due to the high affinity, Ni-NTA ..
Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni2+) for the purification of 6x histidine tagged proteins.   This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linke..
The Ni-NTA Resin in a high binding, high capacity nickel charged IMAC resin that purifies recombinant proteins with the polyhistidine (6XHis) sequence.  The resin is 6% cross-linked agarose for excellent structural integrity with a high capacity of 20-40μmoles Ni2+/ml resin that results..
Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  The Zinc Chelating Resin is specifically designed for the purificati..
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