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Affinity Purification Resins and Methods

 

An assortment of affinity purification resins for the separation of both tagged and immunoglobulin molecules. A large selection of affinity coupling chemistries are available to isolate your target molecules including carboxyl containing molecules, nucleic acids, glycoproteins, polyclonal antibodies, oxidized sugars and many more.  Efficient Avidin-Streptavidin and Neutravidin purification is simple and affordable with our immobilized biotin and iminobiotin resins. High affinity biotin tagged protein resins are available along with streptavidin binding and elution buffers and reagents for the quantitation of biotin. Calmodulin resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells are available as resin only or in kit form including binding and elution buffers. Immobilized p-Aminophenyl Phosphoryl Choline consists of a phosphoryl choline covalently linked to beaded agarose and is designed for the purification of C-reactive protein from plasma, ascites and other biological fluids. A more economical substitute for Immobilized p-Aminophenyl Phosphoryl Choline, Immobilized O-Phosphorylethanolamine is also available for the purification of C-reactive protein.  A human recomboninant C-reactive protein is also offered. Purify your GST-tagged or His-tagged proteins with GST-tagged or His-tagged protein purification resins or kits.  Isolate proteins of the hemostatic pathways, growth hormones and growth factors, lipoproteins and other physiological markers with immobilized heparin and lectins.  Rapidly purify lectins, galactose-binding molecules, agglutinins, N-acetylgalactosamine-binding, N-acetylglucasomine-binding and carbohydrate binding molecules with our lectin purification reagents. A variety of non-porous, high antibody binding capacity, cost effective magnetic beads are available. Immobilized boronic acid is designed specifically for the simple isolation of small molecular weight compounds that have cis-diol groups such as ribonucleotide and oligonucleotide RNA. 

 
Download the Protein Purification Handbook for a full list of related products
Download the Purification Resins Handbook for Complete Product List 
Gene/Protein:
Immobilized Reactive Blue 4 is prepared by covalent linking of Reactive Blue-4 dye with 6% cross-linked agarose.  Immobilized Reactive Blue 4 is offered based on property of textile dyes to bind proteins, especially those with affinities to various nucleotides. Furthermore, affinity of rea..
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Gene/Protein:
Immobilized Reactive Red 120 is prepared by covalent linking of Reactive Red 120 dye with 6% cross-linked agarose.  Immobilized Reactive Red 120 is offered based on property of textile dyes to bind proteins, especially those with affinities to various nucleotides. Furthermore, affinit..
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Gene/Protein:
Immobilized Reactive Yellow 3 is prepared by covalent linking of Reactive yellow 3 dye with 6% cross-linked agarose.  Immobilized Reactive Yellow 3 is offered based on property of textile dyes to bind proteins, especially those with affinities to various nucleotides. Further..
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Gene/Protein:
Immobilized S-Hexylglutathione is prepared by covalent coupling of S-Hexylglutathione with 6% cross-linked agarose beads. Immobilized S-Hexylglutathione in used in affinity purification of S-Hexylglutathione binding proteins such as Glutathione S-Transferase.   Click here to request bulk ..
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Gene/Protein:
Immobilized Testosterone is a specialty resin used in purification of Testosterone binding globulins (TBG) from plasma and other samples.   Click here to request bulk or custom sizes.   Features: ​Matrix: 6% cross-linked agarose Extent of labeling: 0.4-0.6 mmol per ml r..
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Gene/Protein:
Immobilized Triton X-100 is designed to allow refolding of recombinant proteins that were aggregated or formed inclusion bodies when expressed in the genetically engineered cells. The refolding of misfolded proteins is adapted from GroE chaperone refolding mechanism. The misfolded proteins or inclus..
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Gene/Protein:
Immobilized Uridine 5′-Triphosphate is an affinity resin used in purification of uridine dependent enzymes such as uridine kinase from Ehrlich ascites carcinoma.   Click here to request bulk or custom sizes. Features: UTP  coupled to 6% cross-linked Agarose via spacer ..
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Gene/Protein:
Immobilized Vitamin B12 is designed for affinity purification of Vitamin B12 binding proteins. Click here to request bulk or custom sizes.   Features: Matrix: 6% cross-linked agarose Extent of labeling: 0.8-1.2 mg/ml resin Storage buffer: 20% ethanol   Appli..
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Gene/Protein:
Immobilized Wheat Germ Agglutinin (WGA) is prepared by covalently linking WGA to the 6% cross-linked agarose. Immobilized Wheat Germ Agglutinin is affinity resin used in purification of serum and membrane glycoproteins as wheat can bind oligosaccharides containing terminal N-acetylgluosamine or chit..
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Gene/Protein:
Immobilized Xanthine is prepared by covalent coupling of Xanthine with 6% cross-linked agarose beads. Immobilized Xanthine is used in affinity purification of Xanthine binding enzymes such as urate oxidase. Click here to request bulk or custom sizes. Features Xanthine coupled to 6% cross..
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Gene/Protein:
Immobilized α-Casein is made by covalently coupling α-Casein with 6% cross linked agarose beads. Immobilized α-Casein has several applications such as use in affinity purification of protein kinases and DNA dependent RNA polymerases. It is also used in protein binding assays. &n..
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Gene/Protein:
Immobilized α-Lactose is prepared by covalent coupling of α-Lactose with 6%cross-linked agarose. It is used in affinity purification of α-Lactose binding proteins such as lectin from Arachis hypogaea. Click here to request bulk or custom sizes. Features: α-Lactose..
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