G-Trap™ Ni IDA Agarose Fast Flow

G-Trap™ Ni IDA Agarose Fast Flow columns are ready to use prepacked affinity columns used for purification of proteins based on immobilized metal ion affinity chromatography (IMAC).

IMAC is based on interaction of cations of transition metals such as Ni2+, Co2+ with protein residues histidine, cysteine and to some extent to tryptophan. In G-Trap™ Ni IDA Agarose Fast Flow , Ni²⁺ are immobilized on cross-linked 6% agarose using iminodiacetic acid groups (IDA) which are covalently bound to resin. 

G-Trap™ Ni IDA Agarose Fast Flow  are specifically designed for purification of recombinant proteins fused with 6x histidine (6XHis) tag.

Other G-Trap™ IDA Agarose Fast Flow column available in 1 ml and 5 ml column volume is G-Trap™ Co IDA Agarose Fast Flow. One can chose between appropriate column as per requirement. 

The properties of G-Trap™ Ni IDA Agarose Fast Flow resin and the Column are listed below.

G-Trap™ Ni IDA Agarose Fast Flow resin

Matrix: Cross-linked agarose beads, 6%

Mean particle size: 90 μm

Chelating Agent: Iminodiacetic acid

Active group: Ni²⁺

Metal ion  density: 20-40 μmol /ml

Binding Capacity: 5-10mg His-tagged protein/ml medium

pH stability Working Range: 3-12

pH stability Cleaning-in-Place (CIP): 2-14

Maximum Flow Velocity: 2-14

Recommended Flow rate: 1 ml/min and 5 ml/min for 1 ml and 5 ml column respectively

Exclusion limit(globular proteins): 4 x 106

Chemical Stability: Stable to all commonly used aqueous buffers: 8 M urea, 6 M guanidine hydrochloride

Storage Conditions: 2 to 8°C, 20% Ethanol

G-Trap™ Ni IDA Agarose Fast Flow​, 1 ml columns

Column Volume: 1 ml

Columns Dimension: 0.7 x 2.5 cm

Column Harware Pressure Limit: 0.5 MPa

Column Hardware: Polypropylene (Biocompatible)

G-Trap™ Ni IDA Agarose Fast Flow, 5 ml columns

Column Volume: 5 ml

Columns Dimension: 1.6 x 2.5 cm

Column Harware Pressure Limit: 0.5 MPa

Column Hardware: Polypropylene (Biocompatible)

Application(s):

• G-Trap™ Ni IDA Agarose Fast Flow columns are ready to use prepacked affinity columns used for purification of proteins based on immobilized metal ion affinity chromatography (IMAC). G-Trap™ Ni IDA Agarose Fast Flow  are specifically designed for purification of recombinant proteins fused with 6x histidine (6XHis) tag.