G-Trap™ G-Acryl Desalting Columns

G-Trap™ G-Acryl Desalting columns are ready to use prepacked columns for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography. G-Trap™ G-Acryl Desalting columns are mostly used for removal of salt or buffer exchange before or after different chromatographic steps. G-Trap™ G-Acryl Desalting columns can be used for separation of proteins based on their sizes.

G- Biosciences offer three types of desalting columns based on different exclusion limit, namely G-Trap™ G-Acryl 600 Desalting Column, G-Trap™ G-Acryl 100 Desalting Column and G-Trap™ G-Acryl 1200 Desalting Column. The characteristics of G-Trap™ G-Acryl Desalting column resin are shown below in table 2

The G-Trap™ G-Acryl columns consist of porous polyacrylamide beads that are extremely hydrophilic and essentially free of charge. The resin is compatible with the following reagents:

• Dilute Organic Acids
• Urea [8M]
• Guanidine-HCl [6M]
• Chaotropic Agents
• Reducing Agents (DTT, 2-mercaptoethanol)
• Detergents (SDS, CHAPS, Triton® X-100, etc)
• Alcohols (<20%)
• Formamide

The G-Trap™ columns are made of biocompatible polypropylene, which does not interact with biomolecules. The column has a stopper at the inlet and snap-off end at the outlet. The characteristics of the column are listed in Table1.

Table 1: G-Trap™ G-Acryl Desalting Column

NOTE: The pressure over the packed volume varies depending upon the type of medium or matrix, sample or liquid viscosity, and the column tubing used.

Table 2: G-Trap™ G-Acryl Desalting Column resin