G-Sep™ Agarose 4B

G-Sep™ Agarose 4B is a gel filtration matrix formed from agarose beads that is available with 4% agarose content, designated G-Sep™ Agarose 4B.

The resin is a proven gel filtration base matrix and is routinely modified by researchers to couple affinity ligands.  The resin is not pre-activated.

Our G-Sep™ Agarose is also available with 6% agarose content, designated G-Sep™ Agarose 6B.

G-Sep™ Agarose CL resins are cross-linked versions of our G-Sep™ Agarose. Cross-linking of the agarose results in chemically and physically more stable agarose beads that offer the same selectivity, but with better flow characteristics. Cross-linked agarose beads are resistant to organic solvent.

Features

• Matrix: 4% agarose
• Bead form: Spherical, diameter 50-160µm
• pH stability: 4-9
• pH stability cleaning-in-place (CIP): 4-9
• Maximum pressure (MPa): 0.008
• Maximum flow velocity: 11cm/h
• Fractionation [Mr] globular proteins: 6x10⁴ - 2x10⁷
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Chemical stability: 6M urea, 8M guanidine hydrochloride
• Sterilization: Chemical
• Storage condition: 4-30ºC, 20% ethanol

Applications

• Gel filtration media
• Base matrix for coupling affinity ligands